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GAPDH Monoclonal antibody
经过测试的应用| Positive WB detected in | HeLa
cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish
tissue, whole yeast, whole Nematode tissue, soybean whole plant
tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells,
K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells,
PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain
tissue | | Positive IP detected in | HeLa Cells | | Positive IF detected in | Ethacrynic acid treated HeLa cells | | Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody. 推荐稀释比| Application | Dilution |
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| Western Blot (WB) | WB : 1:50000-1:500000
| | Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
| | Immunofluorescence (IF) | IF : 1:200-1:2000
| | Sample-dependent, check data in validation data gallery |
产品信息60004-1-Ig targets GAPDH in WB, IP,
IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with
human, mouse, rat, yeast, plant, zebrafish samples. | Tested Applications | FC, IF, IP, WB, ELISA | | Cited Applications | ChIP, CoIP, FC, IF, IHC, IP, WB | | Tested Reactivity | human, mouse, rat, yeast, plant, zebrafish | | Cited Reactivity | Arabidopsis,
Bombyx mori, bovine, Branchiostoma belcheri, brovine, Caenorhabditis
elegans, canine, chicken, CHO cell line, Cynomorium songaricum | | Immunogen | GAPDH fusion protein Ag0766 种属同源性预测
| | Host / Isotype | Mouse / IgG2b | | Class | Monoclonal | | Type | Antibody | | Full Name | glyceraldehyde-3-phosphate dehydrogenase | | Synonyms | G3PD, GAPD, GAPDH, OK/SW cl.12 | | Calculated molecular weight | 36 kDa | | Observed molecular weight | 36 kDa | | GenBank accession number | BC004109 | | Gene symbol | GAPDH | | Uniprot | P04406 | | Gene ID (NCBI) | 2597
| | RRID | AB_2107436 | | Conjugate | Unconjugated | | Form | Liquid | | Purification Method | Protein A purification | | Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. | | Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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