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Mouse IL-1 beta ELISA Kit

规格
  1. 96T
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产品编号 KE10003
  • 商品说明

Product Information

KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL1-beta. The assay recognizes mouse IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL1-beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL1-beta is added to detect the captured IL1-beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.NB: precursor and active form can be detected by this kit.

Product nameMouse IL-1 beta ELISA Kit
Tests1 X 96 well plate
Sample typeSerum, Plasma, Cell culture supernatants, tissue lysates
Assay typeSandwich
Sensitivity1.0 pg/mL
Range7.8-500 pg/mL
ReactivityMouse
Tested applicationsSandwich ELISA
Gene ID (NCBI)16176

Recovery

Sample TypeAverageRange
Mouse serum102%91%-123%
Cell culture supernatants113%89%-126%
Cell lysates104%82%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120520.017.03.3
220159.55.63.5
32029.10.51.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124485.520.14.1
224156.64.83.1
32428.51.34.6

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Properties

Storage InstructionsAll the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
SynonymsIl 1b, IL 1beta, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1b, IL1beta, IL1β, interleukin 1 beta


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