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产品编号 KE10008
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| Product name | Mouse IL-10 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16153 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 84% | 77%-92% |
| Cell culture supernatants | 104% | 83%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,002.3 | 39.8 | 4.0 |
| 2 | 20 | 280.0 | 6.8 | 2.4 |
| 3 | 20 | 78.2 | 5.1 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,000.7 | 61.1 | 6.1 |
| 2 | 24 | 330.5 | 25.6 | 7.7 |
| 3 | 24 | 99.3 | 8.7 | 8.7 |
Background Information
Interleukin-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class IL antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSIF, Il 10, Il10, IL-10, interleukin 10, Interleukin-10 |
